Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3165582 | Oral Oncology | 2008 | 8 Pages |
Abstract
Anti-tumor immunity plays an important role in the development of and protection from malignancy. However, there is a lack of information regarding induction of CD4+ T helper responses in patients with squamous cell carcinoma (SCCHN). To explore anti-tumor immune responses against SCCHN, a permanent cell line, Gun-1 was established from a squamous cell carcinoma of the hypopharynx. In addition to its characterization, we performed mixed lymphocyte-tumor cell cultures (MLTC) using peripheral blood lymphocytes and autologous tumor cells. Furthermore, T cell responses to wild type (wt) p53-derived peptides were assessed. Gun-1 cells overexpressed p53 and were negative for HLA-A2 expression. No tumor-specific or wt p53-specific CD8+ CTL lines could be established from peripheral blood mononuclear cells (PBMCs) of this patient. Autologous tumor-specific HLA-DR-restricted CD4+ T helper clone was obtained by limiting dilutions using bulk populations from MLTC. This clone produced IFN-γ but not IL-5 in response to autologous tumor cells. In addition, CD4+ T cells were generated from the patient's PBMCs which responded to two HLA-DP5-restricted wt p53-derived peptides. Our results suggest that the immune cells specific for autologous tumor as well as wt p53-derived epitopes are present in the peripheral circulation of this cancer patient. However, helper-type CD4+ T lymphocytes represent the predominant anti-tumor response.
Keywords
IVST helper type 1In vitro stimulationEBV-BT helper type 2MLTCProstaglandin E-2PGE-2SCCHNTh2Th1PBLsMitomycin Cphorbol 12-myristate 13-acetateTGFPBMCsDCsmAbFBSp53MMCCTLsPMAMonoclonal antibodyhuman leukocyte antigenstumor-associated antigensHLAinterferonIFNinterleukintransforming growth factorTumor-infiltrating lymphocytesTILSTAAsfetal bovine serumperipheral blood mononuclear cellsDendritic cellsCD4+ T cellsCytotoxic T lymphocytesperipheral blood lymphocytesmajor histocompatibility complexMHCwild typeSquamous cell carcinoma of the head and neckcomplete medium
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Authors
Kazuaki Chikamatsu, Koichi Sakakura, Takanori Yamamoto, Nobuhiko Furuya, Theresa L. Whiteside, Keisuke Masuyama,