| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3168298 | Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology | 2009 | 6 Pages |
ObjectiveConditioned media (CM) from human dental pulp cells (HDPC) was investigated for its effects on the proliferation and differentiation of HDPC and MG63 cells.Study designCM prepared from the primary culture of HDPC was used for the culture of HDPC and MG63. Cell growth, alkaline phosphatase (ALPase) activity, mRNA expression of differentiation-related genes, and mineralization of the HDPC and MG63 cells in the media containing CM were assessed.ResultsHDPC CM increased the cell growth of HDPC but decreased MG63 cell growth. ALPase activity and the mineralization of both HDPC and MG63 were enhanced by HDPC CM. The CM also up-regulated the expressions of DSPP, DMP-1, and OCN mRNA in HDPC. Pretreatment of HDPC CM with a neutralizing antibody against TGF-β completely eliminated the effect of CM on ALPase activity in HDPC.ConclusionHDPC was able to secrete odontogenic differentiation-inducing factors, in which TGF-β seems to a key element of the CM effects.
