Effect of a calcium hydroxide–based paste associated to chlorhexidine on RAW 264.7 macrophage cell line culture

ObjectiveThe objective of this study was to evaluate the effect of a calcium hydroxide Ca(OH)2-based paste (Calen) associated or not to 0.4% chlorhexidine digluconate (CHX) on RAW 264.7 macrophage cell line culture.Study designThe cell viability (MTT assay), immunostimulating properties (NO dosage), and anti-inflammatory properties (NO, TNF-α, and IL-1α dosage) were evaluated after cell exposure to the materials. Data were analyzed statistically by Kruskal-Wallis test at 5% significance level.ResultsThere was low immunostimulating activity of the Calen paste associated or not to 0.4% CHX in the different materials' concentrations evaluated (P > .05). Anti-inflammatory activity with inhibition of NO and cytokine (TNF-α and IL1-α) release was observed only with Ca(OH)2 + CHX at the highest concentration (25 μg/mL).ConclusionAs the Calen paste associated to 0.4% CHX did not alter cell viability or the immunostimulating and anti-inflammatory properties, the addition of CHX brought no benefits to the Ca(OH)2-based paste with regard to the tested parameters.