| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3168891 | Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology | 2008 | 11 Pages |
ObjectiveThis study was designed to compare mesenchymal stem cell (MSC)-based alveolar bone regeneration in biphasic bone substitutes and natural bone mineral in a canine full-thickness alveolar defect model.Materials and methodsMSCs were isolated from bone marrow aspirates and culture expanded through 3 successive subcultures. The bone differentiation potential of third passage cells was evaluated and confirmed in vitro before cells were used in the transplantation experiment. Undifferentiated cells were then incubated with 3 × 3 × 3 mm3 hydroxyapatite/β-tricalcium phosphate (HA/TCP) matrices (Kasios, Lanauguet, France) and 1- to 2-mm Bio-Oss spongiosa (Geistlich Biomaterials, Osteohealth, Switzerland), which is a natural bovine bone mineral (NBM). Kasios/cell, Kasios alone, Bio-Oss/cell, and Bio-Oss alone were implanted in masseter muscle and 4 cylindrical (10-mm diameter) through-and-through bilateral mandibular body defects in 4 mongrel dogs. Histomorphometric analysis was performed 6 weeks after insertion of the scaffold loaded with MSCs.ResultsH&E staining of the decalcified scaffold and scanning electron microscopy demonstrated large MSC coverage of the HA/TCP and Bio-Oss. Cell-loaded Kasios matrices showed the greatest amount of the bone regeneration among the groups in both the muscle (29.11%) and the bone specimens (65.78%). Cell-free biphasic scaffold revealed 44.9% bone fill in bone defects and 23.55% in muscle specimen, and Bio-Oss alone matrices had the least amount of new bone formation: 36.84% and 24.16% in bone and muscle specimens respectively. Kasios loaded with MSCs demonstrated more bone regeneration than Bio-Oss/cell but there was no significant statistical difference (P > .05).ConclusionsNew biphasic synthetic bone substitutes may offer better conditions for bone regeneration than traditional bone substitute in combination with MSCs. They remained in the defect and contributed to bone regeneration.
