Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3179706 | Tanta Dental Journal | 2013 | 10 Pages |
BackgroundThe aim of our study was to evaluate the effect of local application of melatonin gel on the healing of surgically induced periodontal osseous defects in diabetes-induced rabbits.Material and methodsDiabetes induction was done for 28 rabbits, then, acute periodontal osseous defects were done. Rabbits were divided into: group I: periodontal defects filled with collaplug saturated with placebo gel and group II: periodontal defects filled with collaplug saturated with 2 mg melatonin gel. The expression of RANKL m RNA in the gingival biopsies was examined by real time RT-PCR. Histological and histomorphometric evaluation were performed for both groups at 3 and 6 weeks.ResultsThe levels of RANKL mRNA expression were significantly increased at 2 weeks after diabetes induction. Then, ten days after application of melatonin gel, the level of expression of RANKL significantly decreased as compared to control group. Histologically and histomorphometrically, there was a high significant increase in the percentage of new bone formation and the number of osteoblasts in melatonin-treated group as compared to control group. The number of osteoclasts significantly decreased in melatonin-treated group in both examination periods.ConclusionThe local application of 2 mg melatonin gel offers a promising therapeutic approach for an efficient bone and PDL regeneration.