| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3257061 | Clinical Immunology | 2012 | 8 Pages |
Expression of transcription factor Fli-1 is implicated in the development of glomerulonephritis. Fli-1 heterozygous knockout (Fli1+/−) NZM2410 mice, a murine model of lupus, had significantly improved survival and reduced glomerulonephritis. In this study, we found that infiltrated inflammatory cells were significantly decreased in the kidneys from Fli-1+/− NZM2410 mice. The expression of monocyte chemoattractant protein-1 (MCP-1) was significantly decreased in kidneys from Fli-1+/− NZM2410 mice. The primary endothelial cells isolated from the kidneys of Fli-1+/− NZM2410 mice produced significantly less MCP-1. In endothelial cells transfected with specific Fli-1 siRNA the production of MCP-1 was significantly reduced compared to cells transfected with negative control siRNA. By Chromatin Immunoprecipitation (ChIP) assay, we further demonstrated that Fli-1 directly binds to the promoter of the MCP-1 gene. Our data indicate that Fli-1 impacts glomerulonephritis development by regulating expression of inflammatory chemokine MCP-1 and inflammatory cell infiltration in the kidneys in the NZM2410 mice.
► Fli-1 regulates expression of MCP-1 in the endothelial cells in kidneys. ► Less inflammatory cells infiltrate in the kidneys with reduced expression of Fli-1. ► Fli-1 directly binds to the promoter of the MCP-1 gene. ► Fli-1 impacts glomerulonephritis development by regulating expression of MCP-1.
