Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3258200 | Clinical Immunology | 2007 | 8 Pages |
Abstract
In the present study, we developed a HLA class II tetramer-based detection system utilizing DRB1â0402 tetramers loaded with recently identified immunodominant peptides of desmoglein 3 (Dsg3), the major autoantigen of pemphigus vulgaris (PV). Initial experiments demonstrated staining of a Dsg3-reactive T cell hybridoma which was derived from HLA-DR0402-transgenic mice with loaded PE-labeled DRβ1â0402 tetramers. However, staining of autoreactive T cell clones (TCC) derived from PV patients resulted only in positive staining by addition of exogenous peptides to the staining reactions. There was a dose-dependent specific binding of TCC to the tetramers with the agonistic Dsg3 peptide which was not altered by exogenous unrelated Dsg3 peptide. Noteworthy, the TCC did not stain with HLA-DR4 tetramers complexed with unrelated Dsg3 peptides. The findings of this study suggest that HLA class II tetramers may provide a highly specific approach to monitor ex vivo the T cellular autoimmune response against Dsg3 in patients with PV.
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Authors
Christian Veldman, Rüdiger Eming, Sonja Wolff-Franke, Grete Sonderstrup, William W. Kwok, Michael Hertl,