Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3258392 | Clinical Immunology | 2008 | 9 Pages |
Abstract
Churg Strauss Syndrome (CSS) is a systemic vasculitis in which oligoclonal T cell expansions might be involved in the pathogenesis. Combined analysis of TCR-Vβ expression profile by flow cytometry and of TCR gene rearrangement by heteroduplex PCR was used to detect and characterize T cell expansions in 8 CSS patients, 10 asthmatics and 42 healthy subjects. In all CSS patients one or two Vβ families were expanded among CD8+ cells, with an effector memory phenotype apt to populate tissues and inflammatory sites. Heteroduplex PCR showed the presence of one or more clonal TCR rearrangements, which reveals monoclonal or oligoclonal T cells subpopulations. After purification with a Vβ specific monoclonal antibody, each CD8+/Vβ+ expanded family showed a single TCR rearrangement, clearly suggestive of monoclonality. All CD8+ expansions were detectable throughout the disease course. TCR-Vβ expanded or deleted populations were not observed in asthmatic patients. Clonal CD8+/Vβ+ T cell expansions might be useful as a disease marker.
Related Topics
Life Sciences
Immunology and Microbiology
Immunology
Authors
Giuseppe Guida, Antonella Vallario, Stefania Stella, Monica Boita, Paola Circosta, Sara Mariani, Giuseppina Prato, Enrico Heffler, Roberta Bergia, Antonino Sottile, Giovanni Rolla, Alessandro Cignetti,