| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3270427 | International Journal of Diabetes Mellitus | 2010 | 5 Pages |
IntroductionType 2 diabetes mellitus (T2DM) affects huge populations in India and abroad. Genetic polymorphisms (SNPs) in scavenger receptors such as CD36 have been implicated in the pathogenesis of diabetic atherosclerosis and cardiovascular diseases. Since CD36 gene expression contributes to T2DM, we proposed to study the association of two of its polymorphisms.MethodsA population of 400 subjects from North India was analyzed according to clinical parameters. Out of them 150 controls and 250 T2DM patients were genotyped for two SNPs namely rs1761667 (G > A) and rs1527483 (C > T) in the CD36 gene using polymerase chain reaction and restriction fragment length polymorphism (PCR–RFLP) followed by statistical analysis.Results and discussionNo association of rs1527483 (C > T) SNP was observed in T2DM patients. The GA genotype was prevalent in 76.0% diabetic population and a highly significant genotypic association of rs1761667 (G > A) SNP in CD36 gene was observed in them (P = <0.001; OR 3.173; CI 1.098–9.174). Sample characteristics showed a highly significant association with lipid profile (P = <0.001). The ‘GA’ genotype in combination with CC genotype showed a significant association with TC (P = 0.020), LDL (P = 0.005) and VLDL (P = 0.029). In addition, the haplotype analysis CC/GA (−/+) and CT/GA (−/+) showed a strong association with TC and LDL (P < 0.05). The presence of −31118 A∗ allele in haplotypes showed strong association with T2DM (P = <0.005).ConclusionOut of the two CD36 gene polymorphisms tested, rs1761667 SNP is significantly associated with T2DM with the GA heterozygous genotype showing highest frequency among the T2DM patients.
