Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3293666 | Gastroenterology | 2013 | 9 Pages |
Abstract
Despite the prevalence of hepatitis C virus genotype 4, no replicon system is available for study of the genotype. To facilitate discovery and development of reagents against this virus, we synthesized and transcribed a genotype 4a subgenomic replicon and transfected Huh7-Lunet cells with it, which yielded very few colonies. However, when we used a new Huh-7-derived cell line, colony formation increased â¼70-fold. We identified multiple adaptive mutations in the virus's nonstructural 3 or 4A proteins that allowed the cells to maintain stable, genotype 4a luciferase-encoding replicons. Several classes of hepatitis C virus inhibitors had different antiviral effects on genotypes 4a vs 1b. The genotype 4a replicon system we created will aid in the development of treatment regimens for all genotypes of hepatitis C virus.
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Authors
Betty Peng, Mei Yu, Simin Xu, Yu-Jen Lee, Yang Tian, Huiling Yang, Katie Chan, Hongmei Mo, John McHutchison, William IV, Guofeng Cheng,