Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3335900 | Transfusion and Apheresis Science | 2010 | 9 Pages |
Abstract
Quality control is essential to validate extracorporeal photopheresis (ECP) processes. There is just one protocol based on PHA-induced proliferation. Since it involves the use of radioactive thymidine, we developed another technique using CFSE labeling. We compared the two tests in a paired series including 18 procedures. The thymidine test was valid. Once proliferation was obtained (10 patients out of 13), the CFSE test was in close agreement with it. In particular, two cases of residual proliferation after ECP were simultaneously detected by both techniques. Only the CFSE test allows targeted analysis of lymphocytes, thus identifying a surviving lymphocytic sub-population.
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Authors
Bertrand Evrard, Annie Dosgilbert, Nathalie Jacquemot, François Demeocq, Thibault Gilles, Jacques Chassagne, Marc Berger, Arlette Tridon,