| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3336416 | Transfusion and Apheresis Science | 2006 | 8 Pages |
Abstract
One of the important clinical variables determining the success of hematopoietic stem cell transplantation is the number of viable CD34+ stem cells transfused to the patient. G-CSF mobilized peripheral blood stem cells from 17 healthy donors were collected by continuous flow apheresis. The median (range) proportions of early apoptotic (Annexin V-FITCpos/7-AADneg) and viable (Annexin V-FITCneg/7-AADneg) CD45dimCD34+ stem cells were 1.5 (0.9-3.7)% and 97.7 (82.8-100)% in the peripheral blood before apheresis and 2.6 (0.8-7.9)% and 97.3 (91.9-99)% in the apheresis products, respectively. Despite an increase in the number of apoptotic cells among all cell compartments, this was statistically significant only in CD34+ cells and granulocytes. The majority of the cells still retained their viability.
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Authors
Sule Mine Bakanay, Klara Dalva, Mutlu Arat, Onder Arslan, Erol Ayyildiz, Meral Beksac, Osman Ilhan,