Practical Laboratory Aspects of Cystic Fibrosis Microbiology: an Update, Part I

Cystic fibrosis (CF) microbiology occupies a unique niche in the clinical microbiology laboratory. It is notable for the diversity of potential pathogens, the labor-intensive nature of culture evaluation, and the complexity of organism identification and susceptibility testing. This article provides an update of the major CF pathogens and offers a practical laboratory guide that addresses some of the issues encountered with these cultures. Part I of this two-part article reviews the new and most current information gathered from the literature since 2006 with respect to Pseudomonas aeruginosa, Burkholderia cepacia complex, and other glucose non-fermenting gram-negative rods. Part II of this article will complete the review of the key organisms associated with CF infections, including Staphylococcus aureus, nontuberculous mycobacteria, and fungi. Issues regarding susceptibility testing will also be addressed. Finally, a practical laboratory guide will be provided to address some of the confounding issues associated with CF microbiology.