Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3347482 | Diagnostic Microbiology and Infectious Disease | 2011 | 5 Pages |
Abstract
To analyze 67 clinical methicillin-resistant Staphylococcus aureus (MRSA) isolated from pediatric hospital infections, we used multilocus variable-number tandem-repeat DNA sequence-based techniques, targeting the protein A polymorphic X region and the clumping factor B complete R domain. We define a “clfB similarity score” and then compare the double loci analysis of closely related MRSA isolates with pulsed-field gel electrophoresis (PFGE). We found an endemic clone (MLST-ST8, spa-t008, SCCmecIV, ClfB lineage 1) able to originate 3 possible outbreaks and a second clone (MLST-ST152, spa-t355, SCCmecV, ClfB lineage 4) responsible for limited cases of MRSA infections, indicating that the combination of spa and clfB-lineage typing is useful to trace MRSA pediatric outbreaks.
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Authors
Elisabetta Ugolotti, Roberto Bandettini, Anna Marchese, Laura Gualco, Irene Vanni, Luana Borzi, Eddi Di Marco, Elio Castagnola, Giovanni Melioli, Roberto Biassoni,