| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3347599 | Diagnostic Microbiology and Infectious Disease | 2009 | 7 Pages |
Abstract
Yersinia pestis isolates were genotyped analyzing the polymorphic DNA regions named variable number tandem repeats (VNTR). Allele variants were studied by high-resolution melting analysis (HRMA) of polymerase chain reaction fragments obtained for 25 VNTR loci. After comparison with previous results, 14 loci gave distinguishable normalized melting curves and allowed to correctly assign alleles. This HRMA typing technique permits to differentiate Y. pestis isolates and turned out to be robust, reproducible, and cheap.
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Authors
Andrea Ciammaruconi, Saverio Grassi, Giovanni Faggioni, Riccardo De Santis, Valentina Pittiglio, Raffaele D'Amelio, Gilles Vergnaud, Florigio Lista,