Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3347609 | Diagnostic Microbiology and Infectious Disease | 2009 | 4 Pages |
Abstract
We report a TaqMan® real-time polymerase chain reaction (PCR) protocol for diagnosing cryptococcosis. Specificity was tested with 33 fungal strains. The 7 clinical samples found positive by culture also tested positive by real-time PCR. No false negatives were found among the 94 clinical samples that were negative by culture for Cryptococcus spp. The sensitivity threshold was about 10 plasmid copies per assay.
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Authors
Vincent Veron, Stephane Simon, Denis Blanchet, Christine Aznar,