Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3347727 | Diagnostic Microbiology and Infectious Disease | 2009 | 6 Pages |
Abstract
Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.
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Authors
Carson D. Baldwin, Gerald B. Howe, Ranga Sampath, Larry B. Blyn, Heather Matthews, Vanessa Harpin, Thomas A. Hall, Jared J. Drader, Steve A. Hofstadler, Mark W. Eshoo, Karl Rudnick, Karen Studarus, David Moore, Sharon Abbott, J. Michael Janda,