Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3347812 | Diagnostic Microbiology and Infectious Disease | 2011 | 9 Pages |
Abstract
Thermostable direct hemolysin encoded by tdh gene has been considered an important virulence factor in pathogenic Vibrio parahaemolyticus. Two-step ultrarapid real-time polymerase chain reaction (URRT PCR) with a microchip was devised to detect V. parahaemolyticus carrying tdh gene. This novel method has a 6-μL reaction volume and extremely reduces running time since one cycle can be completed in 10 s or less. Consequently, 35 cycles of URRT PCR was successfully able to detect up to 100 fg (18 copies) of genomic DNA from pathogenic V. parahaemolyticus carrying tdh gene in 6 min. These results indicate that this method is at present the most rapid detection method for tdh gene and pathogenic V. parahaemolyticus.
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Authors
Min-Hee Kang, Il-Wook Kim, Dong-Woo Lee, Mi-Sun Yoo, Sang-Hoon Han, Byoung-Su Yoon,