Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3348134 | Diagnostic Microbiology and Infectious Disease | 2008 | 4 Pages |
Abstract
Rapid and accurate tests capable of distinguishing Bordetella pertussis from milder Bordetella parapertussis infection can aid in treating patients. We evaluated a novel real-time polymerase chain reaction (PCR) method that allows rapid and accurate diagnosis and distinction between B. pertussis and B. parapertussis infections. The method is based on a fluorescent 5â²-minor groove-binding molecule hybridization probe that can clearly distinguish between B. pertussis and B. parapertussis by post-PCR melt curve analysis. The reagents worked equally well in several different real-time PCR instruments. The Bordetella detection reagents are combined with internal control components to account for PCR inhibition without compromising assay sensitivity.
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Authors
Irina Afonina, Mark Metcalf, Alan Mills, Walt Mahoney,