| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3348159 | Diagnostic Microbiology and Infectious Disease | 2007 | 4 Pages |
Abstract
Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Applied Microbiology and Biotechnology
Authors
Karen Orjuela, Catalina Amador, Manuel A. Patarroyo,