Article ID Journal Published Year Pages File Type
3348315 Diagnostic Microbiology and Infectious Disease 2008 4 Pages PDF
Abstract

The antimicrobial activities of 6 common antimicrobial agents including carbapenems were tested against 100 clinical Pseudomonas aeruginosa isolates using a disk diffusion method. All imipenem (IPM)-resistant isolates were screened for metallo-β-lactamase (MBL) production by Etest assay and were subsequently subjected to polymerase chain reaction (PCR) analysis with the blaIMP and blaVIM genes. Of 41 IPM-nonsusceptible isolates detected, 8 (19.51%) appeared to produce MBL, as determined by Etest. Using PCR assay, these isolates were positive for blaVIM genes, whereas none were positive for blaIMP genes.

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