| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3348747 | Diagnostic Microbiology and Infectious Disease | 2007 | 4 Pages |
Abstract
We developed a novel rapid assay to detect the gyrA mutations that cause quinolone resistance in typhoid and paratyphoid fever Salmonella spp. using high-resolution melting (Idaho Technology, Salt Lake City, UT) analysis of polymerase chain reaction amplicons. The presence of gyrA mutations led to small but consistent changes in amplicon melting temperatures that allowed quinolone-resistant isolates to be differentiated from susceptible ones.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Applied Microbiology and Biotechnology
Authors
Robert Slinger, Deana Bellfoy, Marc Desjardins, Francis Chan,