Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3352363 | Human Immunology | 2006 | 9 Pages |
Abstract
We present here the complete coding sequences, previously unavailable, of the DRB1 alleles DRB1*030102, *0306, *040701, *0408, *1327, *1356, *1411, *1446, *1503, *1504, *0806, *0813, and *0818. For cDNA isolation, new group-specific primers located at the 5â²UT and 3â²UT regions were used to carry out allele-specific amplification and a convenient method for determining full-length sequences for DRB1 alleles. Complete coding sequencing of samples previously typed as DRB1*0406, DRB1*080101, and DRB1*1111 revealed new alleles with noncoding nucleotide changes at exons 1 and 3. In addition, we found a novel allele, DRB1*0113, whose second exon carries a sequence motif characteristic of DRB1*07 alleles. The predicted class II haplotypic associations of all alleles are reported and discussed.
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Immunology and Microbiology
Immunology
Authors
Antonio Balas, Carlos Vilches, Miguel A. RodrÃguez, Begoña Fernández, Maria Paz Martinez, Rosario de Pablo, Félix GarcÃa-Sánchez, Jose L. Vicario,