| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3355693 | Immunology Letters | 2012 | 8 Pages |
Visualizing the in vivo dynamics of individual donor cells after allogeneic hematopoietic stem cell transplantation (HSCT) will enable deeper understanding of the process of graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL). In this study, using non-invasive in vivo fluorescence imaging of the ear pinna, we successfully visualized green fluorescent protein (GFP) donor cells at the single cell level in the skin. This imaging model enabled visualization of the movement of GFP cells into blood vessels in real time after allogeneic HSCT. At day 1, a few donor cells were detected, and the movement of donor cells in blood vessels was readily observed at day 4. Early donor cell infiltration into non-lymphoid tissue was increased by treatment with croton oil, as an inflammatory reagent. Treatment with dexamethasone, as an anti-inflammatory reagent, suppressed donor cell infiltration. The in vivo cellular fluorescence imaging model described here is a very useful tool for monitoring individual donor cells in real-time and for exploring immunomodulatory reagents for allogeneic HSCT, as well as for understanding the mechanism of GVHD.
► We visualized GFP donor cells at the single-cell level in vivo after allogeneic HSCT. ► We non-invasively visualized GFP donor cells in the ear pinna after induction of GVHD. ► Donor cell infiltration was increased by treatment with croton oil. ► Treatment with dexamethasone suppressed donor-cell infiltration. ► In vivo imaging is a useful tool for exploring immunomodulatory drugs for GVHD.
