Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3356359 | Immunology Letters | 2007 | 12 Pages |
Abstract
We have shown previously that a fetal sheep liver extract (FSLE) containing significant quantities of fetal ovine gamma globin chain (Hbγ) and LPS injected into aged (>20 months) mice could reverse the altered polarization (increased IL-4 and IL-10 with decreased IL-2 and IFNγ) in cytokine production seen from ConA stimulated lymphoid cells of those mice. The mechanism(s) behind this change in cytokine production were not previously investigated. We report below that aged mice show a >60% decline in numbers and suppressive function of both CD4+CD25+Foxp3+ Treg and so-called Tr3 (CD4+TGFβ+), and that their number/function is restored to levels seen in control (8-week-old) mice by FSLE. In addition, on a per cell basis, CD4+CD25âTreg from aged mice were >4-fold more effective in suppression of proliferation and IL-2 production from ConA-activated lymphoid cells of a pool of CD4+CD25âT cells from 8-week-old mice than similar cells from young animals, and this suppression by CD25âT cells was also ameliorated following FSLE treatment. Infusion of anti-TGFβ and anti-IL-10 antibodies in vivo altered Treg development following FSLE treatment, and attenuated FSLE-induced alterations in cytokine production profiles.
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Authors
R.M. Gorczynski, C. Alexander, W. Bessler, K. Brandenburg, K. Fournier, J.P. Mach, S. Mueller, E.Th. Rietschel, A.J. Ulmer, Th. Waelli, U. Zahringer, I. Khatri,