| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3360690 | International Journal of Antimicrobial Agents | 2008 | 4 Pages |
The aim of the present study was to determine the occurrence of seven virulence determinants in Enterococcus faecium clinical blood culture isolates over a 6-year period and to investigate possible correlations between virulence and antibiotic resistance. Two hundred and sixty-three isolates were screened for the presence of genes coding for aggregation substance (asa1), cytolysin (cylA), collagen-binding protein (ace), Enterococcusfaecalis endocarditis antigen (efaAfs), enterococcal surface protein (espfm), gelatinase (gelE) and hyaluronidase (hylfm) by polymerase chain reaction. The minimum inhibitory concentrations (MICs) of ampicillin, ciprofloxacin, gentamicin, imipenem, linezolid and vancomycin were determined by the agar dilution method and the MIC of daptomycin was determined by Etest. The espfm gene was found in 56% of the isolates, hylfm in 4%, whilst the other virulence genes were detected only sporadically (≤1%). The level of antibiotic resistance was 77% to ampicillin, 90% to ciprofloxacin and 83% to imipenem; 5% of the isolates were resistant to vancomycin and 2% were resistant to gentamicin (high-level resistance, MIC ≥500 mg/L). A significant correlation was found between the presence of espfm and resistance to ampicillin, ciprofloxacin and imipenem (P < 0.01). Twelve isolates were espfm-positive and ampicillin-susceptible.
