| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3392107 | Transplant Immunology | 2013 | 8 Pages |
The role of specific subtypes of infiltrating cells in acute kidney allograft rejection is still not clear and was so far not examined by different analyzing methods under standardized conditions of an experimental kidney transplantation model.Immunohistochemical staining of CD3, CD20 and CD68 was performed in rat allografts, in syngeneically transplanted rats and in control rats with a test duration of 6 and 28 days. The detailed expression and localization of infiltrating cells were analyzed manually in different kidney compartments under light microscope and by the two different morphometric software programs. Data were correlated with the corresponding kidney function as well as with histopathological classification.The information provided by the morphometric software programs on the infiltration of the specific cell types after renal transplantation was in accordance with the manual analysis. Morphometric methods were solid to analyze reliably the induction of cellular infiltrates after renal transplantation. By manual analysis we could clearly demonstrate the detailed localization of the specific cell infiltrates in the different kidney compartments. Besides infiltration of CD3 and CD68 infiltrating cells, a robust infiltration of CD20 B-cells in allogeneically transplanted rats, even at early time points after transplantation was detected. Additionally an MHC class I expression could reliable be seen in allogeneically transplanted rats. The infiltration of B-cells and the reliable antigen presentation might act as a silent subclinical trigger for subsequent chronic rejection and premature graft loss.
► We established an experimental kidney transplantation model in rats. ► We examined CD3, CD20 and CD68 positive cells under highly standardized conditions. ► The expression of infiltrating cells was analyzed by different analyzing methods. ► Morphometric methods were solid to analyze reliably the induction of infiltrates. ► A robust infiltration of CD20 B-cells in allogeneically transplanted rats was detected.
