| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3392141 | Transplant Immunology | 2012 | 4 Pages |
BackgroundIschemic reperfusion (I/R) injury of the kidney is closely associated with delayed graft function, increased acute rejection, and late allograft dysfunction. Splenectomy reduced hepatic I/R injury by inhibiting leukocyte infiltration in the liver, release of TNF-α, cell apoptosis, and expression of caspase-3. Thus, we investigated the effects of splenectomy on renal I/R injury in the rat.MethodsMale Wistar rats were assigned to four groups: sham operation (sham group), sham operation + splenectomy (sham + SPLN group), right nephrectomy followed by clamping the left renal pedicle for 30 min (I/R 30 group), and I/R 30 + splenectomy (I/R 30 + SPLN group). Renal function was determined by measuring the concentration of blood urea nitrogen (BUN) and serum creatinine (S-Cr). The serum level of tumor necrosis factor-α (TNF-α) was measured as the marker for inflammation. Left kidneys were obtained 24 h after reperfusion. TUNEL assay was assessed for cell apoptosis. Spleens were obtained immediately (0-h group) and 3 h after reperfusion (3-h group). The removed spleens were histologically evaluated.ResultsThe BUN and S-Cr levels were significantly lower in the I/R 30 + SPLN group than in the I/R 30 group (p < 0.05 for both). Apoptotic cells were significantly lower in the I/R 30 + SPLN group than in the I/R 30 group. The serum level of TNF-α, which was increased after I/R, was significantly lower in the I/R 30 + SPLN group than in the I/R 30 group (p < 0.05). Spleen weights were significantly lower in the 3-h group than in the 0-h group (p < 0.05).ConclusionThese results suggest that splenectomy reduces renal I/R injury, and this effect may occur by an anti-inflammatory pathway and inhibition of cell apoptosis.
► The definite role of splenectomy in renal I/R injury has not yet been elucidated. ► We investigated whether splenectomy can reduce renal I/R injury in the rat. ► Splenectomy improved renal function, and histopathological degeneration. ► Splenectomy decreased renal I/R injury damage by inhibiting TNF-α production.
