Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3392517 | Transplant Immunology | 2008 | 10 Pages |
Abstract
It is well documented that ultraviolet (UV) radiation present in sunlight suppresses immune responses. However, the majority of studies documenting the immunosuppressive effects of UV irradiation have been carried out in animals exposed to UV irradiation before immunization. Here, we report that recipient mice exposed to UV irradiation 7Â days after immunization with a donor alloantigen exhibited prolongation of allograft survival in an alloantigen-specific manner. Recipient mice (H-2b) intravenously immunized with 2Â ÃÂ 107 allogeneic spleen cells (H-2b/d) 7Â days before UV irradiation (40Â kJ/m2) showed prolonged survival of allografts presenting the alloantigen used for sensitization (H-2b/d), but not third-party allografts (H-2b/k). Adoptive transfer experiments revealed that CD4+ T cells in UV-irradiated recipients were responsible for this prolongation. CD4+ T cells that could transfer the suppression produced large amounts of interleukin (IL)-10, but not IL-4. The effect of UV irradiation on alloantigen-specific immunosuppression was cancelled by administration of an anti-IL-10 monoclonal antibody. These results indicate that UV irradiation given after alloantigen immunization induces alloantigen-specific type 1 regulatory T cell-like regulatory T cells that prolong allograft survival and imply that the difficulties associated with predicting donor-related organ availability in transplantation can be dealt with, given the effectiveness of UV irradiation after immunization.
Keywords
IFN-γPBSmAbsupernatantsTr1FCSIL-10MLRTregAPCCHSDTHMonoclonal antibodyantigen-presenting cellsUltravioletstandard deviationinterferon-gammainterleukindelayed-type hypersensitivityELISAEnzyme-linked immunosorbent assayToleranceContact hypersensitivityGraft survivalfetal calf serumRegulatory T cellsSupPhosphate-buffered salinenot significantTransplantation
Related Topics
Life Sciences
Immunology and Microbiology
Immunology
Authors
Tomohide Hori, Kagemasa Kuribayashi, Shinji Uemoto, Kanako Saito, Linan Wang, Mie Torii, Shintaro Shibutani, Kentaro Taniguchi, Shintaro Yagi, Taku Iida, Chiduru Yamamoto, Takuma Kato,