Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3405468 | Journal des Anti-infectieux | 2012 | 9 Pages |
Abstract
Until the early 2000s, in the clinical microbiology laboratories, the bacterial identification was based mainly on the biochemical analysis of phenotypic characters whose interpretation required expertise. In most cases, the results was obtained after more than 18Â h. When it was taken in default, the identification was mainly obtained by molecular biology analysis such as 16S rDNA sequences. For nearly 5Â years, using MALDI-TOF mass spectrometry machine revolutionized bacterial identification in clinical bacteriology laboratories. From bacterial colonies, the results of reliable identifications are easily obtained in less than 5Â minutes. These advantages have greatly contributed to its success. When a laboratory is equipped by this device, the bulk of the identifications are provided by this new approach. The objective of this review is to analyze the reasons of the success of MALDI-TOF in clinical bacteriology, to know its present limitations and new applications.
Keywords
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Infectious Diseases
Authors
N. Degand, R. Ruimy,