| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3406583 | Journal of Virological Methods | 2016 | 4 Pages |
Abstract
•Phlebovirus PCR assay targeting both tick- and sandfly/mosquito-borne viruses was designed.•Sensitivity and specificity was tested on a panel of arboviruses.•The assay is simple and affordable for large-scale screening.
This study reports the pan-phlebovirus assay capable of detecting both sandfly/mosquito- and tick-borne phleboviruses. Sensitivity and specificity of the assay was verified using a panel of arboviruses. The RT-PCR assay is simple and sensitive, and thus well suited for screening of field samples.
Keywords
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Virology
Authors
Alexander S. Klimentov, Alexander M. Butenko, Natalia V. Khutoretskaya, Elena Yu. Shustova, Victor F. Larichev, Olga V. Isaeva, Galina G. Karganova, Alexander N. Lukashev, Anatoly P. Gmyl,