Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3407111 | Journal of Virological Methods | 2010 | 6 Pages |
Abstract
A rapid and comprehensive protocol, which combines simple purification and liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI/MS/MS), was developed for identification of arboviruses in infected culture fluid. Using this protocol, various arboviruses were detected including uncommon viruses that were described previously as Banna virus and Yunnan orbivirus. This approach is useful for the rapid screening of viral samples that cannot be identified by conventional gene amplification or immunological methods.
Keywords
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Virology
Authors
Kenta Okamoto, Yushirou Endo, Shingo Inoue, Takeshi Nabeshima, Phan Thi Nga, Posadas H. Guillermo, Fuxun Yu, Do Phuong Loan, Bui Minh Trang, Filipinas F. Natividad, Futoshi Hasebe, Kouichi Morita,