Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3407211 | Journal of Virological Methods | 2009 | 8 Pages |
Abstract
The virus envelope (E) protein of Japanese encephalitis virus induces virus-neutralizing antibodies and is therefore a potential vaccine antigen. In a mammalian system, co-expression of another viral structural protein prM is necessary for proper expression and folding of E protein. Transgenic tobacco plants were produced carrying JEV cDNA encoding prM and E proteins under the control of the CaMV 35S promoter. E protein, however, was not detectable in these plants. In vitro translation studies showed that the presence of the prM sequence inhibited transgene expression in the plant system. Accordingly, JEV E protein could be expressed in transgenic tobacco plants only without the prM protein.
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Authors
Mohan Babu Appaiahgari, M.Z. Abdin, K.C. Bansal, Sudhanshu Vrati,