Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3407303 | Journal of Virological Methods | 2010 | 5 Pages |
Abstract
A recombinant glycoprotein (R-GP) of vesicular stomatitis New Jersey virus (VSV-NJ) was expressed in insect cells by a baculovirus system. Its utility as a diagnostic antigen in a blocking ELISA was investigated as an alternative to the current native GP extracted from VSV-NJ. With the cut-off value of 73% inhibition, the R-GP ELISA exhibited 99.1% specificity for naive sera from cattle and horses. It did not cross-react with VSV-Indiana (VSV-IN) positive sera and differentiated from foot-and-mouth disease and swine vesicular disease. Taken together, this is the first report that the R-GP has a potential to be used as a diagnostic antigen in place of the native GP for the detection of antibodies to VSV-NJ in cattle and horses.
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Authors
Eun-Jeong Heo, Hyang-Sim Lee, Hye-Young Jeoung, Hyo-Rim Ko, Chang-Hee Kweon, Young-Joon Ko,