| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3407733 | Journal of Virological Methods | 2009 | 7 Pages |
Abstract
PCR-enhanced reverse transcriptase assays (PERT) are sensitive tools for the detection of retroviruses in biological samples. The adaptation of real-time PCR techniques based on fluorescent probes (F-PERT) has added a reliable quantitative capacity to the assay. In the interest of economy and time, the SYBR Green I-based real-time detection system was used to establish a convenient one-step PERT assay (SG-PERT). This assay can be completed in 2 h, is linear over six orders of magnitude and can be used to quantify retroviruses belonging to divergent species, such as the human immunodeficiency virus type 1 (HIV-1), murine leukemia virus (MLV) and prototypic foamy virus (PFV).
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Authors
Massimo Pizzato, Otto Erlwein, David Bonsall, Stephen Kaye, David Muir, Myra O. McClure,