Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3407834 | Journal of Virological Methods | 2008 | 4 Pages |
Abstract
A simple, sensitive and specific method using a cDNA macroarray to detect multiple viruses was devised. The method is used in plants such as potato and lily, which need a reliable routine diagnosis for mixed infection. The biotinylated cRNA targets were prepared using an in vitro transcription-based system that was designed especially to eliminate nonspecific hybridizations. The macroarray hybridization was carried out using a convenient, cost-effective “microtube hybridization” (MTH) system. By this method, lily viruses including Cucumber mosaic virus, Lily symptomless virus, Lily mottle virus, and Plantago asiatica mosaic virus were detected successfully from leaves or roots of lily bulbs.
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Authors
Shunpei Sugiyama, Chikara Masuta, Hiroshi Sekiguchi, Taketo Uehara, Hanako Shimura, Yukio Maruta,