Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3407843 | Journal of Virological Methods | 2008 | 4 Pages |
Abstract
A method is described for the use of a G-deleted, conditionally replicating version of vesicular stomatitis virus (VSV) to measure alterations to the innate anti-viral state of cells in vitro. By co-transfecting a gene of interest with an expression vector for VSV-G one can directly measure the replication of the virus in the transfected cells as non-transfected cells will fail to produce infectious progeny due to the lack of glycoprotein in these non-transfected cells. This allows the investigator to focus exclusively on the anti-viral state induced or inhibited in the transfected cells allowing for screening and quantitative analysis of viral or cellular genes that may modify the anti-viral state.
Keywords
Related Topics
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Virology
Authors
Frances Lai, Natasha Kazdhan, Brian D. Lichty,