Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3407982 | Journal of Virological Methods | 2007 | 7 Pages |
Abstract
A TaqMan real-time RT-PCR assay was developed for detection of RNA transcripts produced by replicating CPV-2. A pair of primers and a TaqMan probe targeting the spliced NS2 mRNA were designed. A synthetic DNA fragment was constructed to mimic the spliced NS2 mRNA by PCR-based gene assembly and was used for generation of standard RNAs. The detection limit of the assay was 1 × 102 RNA copies and standard curve displayed a linear range from 1 × 102 to 1 × 109 copies and a good reproducibility. The assay was then applied to determine the mRNA loads in the tissues of dogs naturally infected by CPV-2. mRNA was detected in a variety of tissues, including the central nervous system.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Gabriella Elia, Alessandra Cavalli, Costantina Desario, Eleonora Lorusso, Maria Stella Lucente, Nicola Decaro, Vito Martella, Canio Buonavoglia,