Article ID Journal Published Year Pages File Type
3408024 Journal of Virological Methods 2008 5 Pages PDF
Abstract

A long RT-PCR method was developed to amplify the norovirus genome. Starting from RNA extracted directly from clinical samples and using broadly reactive primers, it can generate near full-length amplicons that allow for easy determination of the near complete genomic sequence. Two norovirus isolates from Toronto, Canada, in 2002 and 2005 were sequenced. This approach will facilitate molecular epidemiology studies of noroviruses.

Related Topics
Life Sciences Immunology and Microbiology Virology
Authors
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