Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3408027 | Journal of Virological Methods | 2008 | 5 Pages |
Abstract
Measles and rubella virus cause fever/rash diseases that are difficult to differentiate clinically. Both viruses can be detected in the same clinical specimens and are propagated on the same cell cultures. A single-tube multiplex TaqMan assay is described for the simultaneous and rapid detection of the full spectrum of known genetic variants. The performance of the assay is similar to a conventional nested PCR and generates cDNA with random primers which can be used directly for virus genotyping.
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Authors
Judith M. Hübschen, Jacques R. Kremer, Sébastien De Landtsheer, Claude P. Muller,