Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3408173 | Journal of Virological Methods | 2007 | 9 Pages |
Abstract
Performance of the Abbott m2000 instrument system and the Abbott RealTime⢠HIV-1 assay was evaluated using a panel of 37 group M (subtypes A-D, F, G, CRF01_AE, CRF02_AG and unique recombinant forms) and 2 group O virus isolates. Testing was performed on 273 sample dilutions and compared to VERSANT HIV-1 RNA 3.0 (bDNA) and AMPLICOR HIV-1 MONITOR v1.5 (Monitor v1.5) test results. RealTime HIV-1, bDNA, and Monitor v1.5 tests quantified 87%, 78%, and 81% of samples, respectively. RealTime HIV-1 detected an additional 31 samples at <40 copies/mL. For group M, RealTime HIV-1 dilution profiles and viral loads were highly correlated with bDNA and Monitor v1.5 values; 87% and 89% of values were within 0.5 log10 copies/mL. In contrast, the group O viruses were not detected by Monitor v1.5 and were substantially underquantified by â¼2 log10 copies/mL in bDNA relative to the RealTime HIV-1 assay. Sequence analysis revealed that RealTime HIV-1 primer/probe binding sites are highly conserved and exhibit fewer nucleotide mismatches relative to Monitor v1.5. The automated m2000 system and RealTime HIV-1 assay offer the advantages of efficient sample processing and throughput with reduced “hands-on” time while providing improved sensitivity, expanded dynamic range and reliable quantification of genetically diverse HIV-1 strains.
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Authors
Priscilla Swanson, Shihai Huang, Klara Abravaya, Carmen de Mendoza, Vincent Soriano, Sushil G. Devare, John Jr.,