Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3408370 | Journal of Virological Methods | 2006 | 5 Pages |
Abstract
We developed a new hybrid nuclear polyhedrosis virus (NPV) bacmid capable of infecting Spodoptera frugiperda, Tricoplusia ni, and Bombyx mori, and B. mori cell lines for producing hybrid recombinant baculovirus that can carry a gene of interest and express it in a broad range of hosts. A GFPuv-β1,3-N-acetylglucosaminyltransferase 2 fusion gene was expressed successfully in silkworm larvae using this hybrid bacmid. The hybrid NPV bacmid provides an altogether simple and realistically feasible method for large-scale applications using silkworm larvae. It can be easily managed in E. coli, which has no biohazard safety concerns, in addition to the baculovirus-based expression system.
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Authors
Vipin Kumar Deo, Masato Hiyoshi, Enoch Y. Park,