| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3408627 | Journal of Virological Methods | 2006 | 5 Pages |
Abstract
The recombinant antigen obtained by cloning and expressing two IBV nucleocapsid protein fragments (143-414 aa, 281-414 aa) in Escherichia coli was used for the detection of avian infectious bronchitis virus (IBV) specific antibodies in chicken sera by the indirect ELISA (rNpIBV-ELISA). As a result of testing 1524 serum samples the diagnostic sensitivity and specificity of rNpIBV-ELISA when comparing those of the routine whole IBV ELISA have been shown to be 93.81% and 87.36%, respectively. The agreement value was 91.5%.
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Authors
N.N. Lugovskaya, A.V. Scherbakov, A.S. Yakovleva, M.A. Tsyvanyuk, N.S. Mudrak, V.V. Drygin, A.V. Borisov,