Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3415002 | Microbes and Infection | 2009 | 8 Pages |
Abstract
Monitoring the spread of mycobacterium in vivo using biophotonic imaging provides a fast, reliable and sensitive method to evaluate the distribution of the infection. Moreover, this technique allows for a significant reduction in the number of animals required in comparison to conventional anatomopathological studies. Here, we describe for the first time and validate the use of a luciferase-tagged recombinant Mycobacterium bovis BCG for non-invasive bioluminescent imaging of 1) bacterial dissemination in tissues, 2) the efficacy of treatment with anti-mycobacterial drugs and 3) the role of adaptive immune responses in controlling mycobacterial infection in vivo.
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Authors
Frank Heuts, Berit Carow, Hans Wigzell, Martin E. Rottenberg,