Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3415332 | Microbes and Infection | 2008 | 9 Pages |
Abstract
The application of Lucilia sericata larvae to chronic, infected wounds results in the rapid elimination of infecting microorganisms, including MRSA. Previously, we demonstrated in vitro antibacterial activity of native excretions/secretions (nES) from L. sericata and partially purified two low mass antibacterial compounds with masses of 0.5-10Â kDa and <500Â Da. The present study reports the antibacterial effects of the <500Â Da fraction (ES<500) on the growth and morphology of a range of bacteria, including 12 MRSA strains. Distinct morphological changes were observed in Bacillus cereus and Escherichia coli following exposure to ES<500. Flow cytometry and confocal microscopy analyses, in conjunction with turbidometric and CFU assays, revealed bacteriostatic activity of nES against S. aureus and E. coli. ES<500 also demonstrated bacteriostatic activity against S. aureus, however, bactericidal activity and the induction of a viable but non-culturable state were observed with ES<500-treated E. coli.
Keywords
PBSCFUnESExcretions/secretionsMWCOTSBCSLMMRSAmethicillin-resistant Staphylococcus aureusstandard error of the meandaltonMaggot therapyTryptic soy brothSurvival IndexAntibacterialPhosphate buffered salineSEMBacterial morphologyConfocal scanning laser microscopylight microscopycolony-forming unitPropidium iodideIndigo carminemolecular weight cut-off
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Authors
Alyson Bexfield, A. Elizabeth Bond, Emily C. Roberts, Edward Dudley, Yamni Nigam, Stephen Thomas, Russell P. Newton, Norman A. Ratcliffe,