Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3415662 | Microbes and Infection | 2009 | 5 Pages |
Abstract
In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28.
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Authors
Feng Qian, Joan A. Aebig, Karine Reiter, Emma Barnafo, Yanling Zhang, Richard L. Shimp Jr., Kelly M. Rausch, David S. Jones, Daming Zhu, Lynn Lambert, Gregory E.D. Mullen, David L. Narum, Louis H. Miller, Yimin Wu,