| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3415684 | Microbes and Infection | 2008 | 5 Pages |
Abstract
To obtain monkey-tropic viruses that are more closely related to HIV-1 than the original NL-DT5/NL-DT5R clones, we constructed six vif-chimeric and two site-specific vif-mutant viruses, and examined their growth ability. Different from NL-DT5/NL-DT5R, these viruses did not grow in monkey cells. We monitored the capability of the mutants to antagonize monkey APOBEC3G/F by single-cycle infectivity assays. They counteracted poorly or not at all the action of the APOBEC3G/F. Our results have indicated that the native SIVmac Vif is required to overcome the species barrier against HIV-1.
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Authors
Kazuki Hatcho, Kazuya Kamada, Tomoki Yamashita, Akio Adachi, Masako Nomaguchi,