| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3417958 | Parasitology International | 2011 | 5 Pages |
ELISA for filaria-specific IgG4 in urine (urine ELISA) was applied to children in 7 schools in Sri Lanka, before and after 5 rounds of annual mass drug administration (MDA). The pre-treatment IgG4 prevalence in 2002 was 3.20%, which decreased to 0.91% in 2003 after the first MDA (P < 0.001), and finally to 0.36% in 2007 after the 5th MDA. Among 5–10 year-old children, the prevalence decreased from 3.37% in 2002 to 0.51% in 2003 (P = 0.009). A pattern of IgG4 titer distribution according to age and its yearly change could also provide useful information in drug efficacy analysis. In 2008, new samples from eleven 2006/07 urine ELISA-positive students and their family members (total n = 56) were examined by ICT antigen test, microfilaria test, and urine ELISA. No infection was confirmed among them. Urine ELISA will be useful in monitoring elimination/resurgence in a post-MDA low endemic situation.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► ELISA for filaria-specific IgG4 in urine was applied to school children in Sri Lanka. ►They were examined before and after 5 rounds of MDA. ► The pre-treatment IgG4 prevalence, 3.20%, decreased to 0.36% after the 5th MDA. ► Yearly changes of IgG4 titer provided useful information in MDA efficacy analysis. ► Urine ELISA will be useful in monitoring extinction/resurgence after MDA.
