| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3424254 | Virology | 2013 | 8 Pages |
Bacteriophage particles contain both simple and complex macromolecular assemblages and machines that enable them to regulate the infection process under diverse environmental conditions with a broad range of bacterial hosts. Recent developments in cryo-electron tomography (cryo-ET) make it possible to observe the interactions of bacteriophages with their host cells under native-state conditions at unprecedented resolution and in three-dimensions. This review describes the application of cryo-ET to studies of bacteriophage attachment, genome ejection, assembly and egress. Current topics of investigation and future directions in the field are also discussed.
► Cryo-ET is used for structural analyses of the bacteriophage replication process. ► Cryo-ET allows for the 3D native-state visualization of bacteriophage and host cells. ► The bacteriophage replication events reviewed highlight developments to cryo-ET. ► Future advancements to the technologies are discussed.
