Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3424436 | Virology | 2012 | 15 Pages |
Recruited host factors aid replication of plus-strand RNA viruses. In this paper, we show that Dbp2 DEAD-box helicase of yeast, which is a homolog of human p68 DEAD-box helicase, directly affects replication of Tomato bushy stunt virus (TBSV). We demonstrate that Dbp2 binds to the 3′-end of the viral minus-stranded RNA and enhances plus-strand synthesis by the viral replicase in a yeast-based cell-free TBSV replication assay. In vitro data with wt and an ATPase-deficient Dbp2 mutant indicate that Dbp2 unwinds local secondary structures at the 3′-end of the TBSV (−)RNA. We also show that Dbp2 complements the replication deficiency of TBSV in yeast containing reduced amount of Ded1 DEAD-box helicase, another host factor involved in TBSV replication, suggesting that Dbp2 and Ded1 helicases play redundant roles in TBSV replication. We also show that the orthologous AtRH20 DEAD-box helicase from Arabidopsis can increase tombusvirus replication in vitro and in yeast.
Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (284 K)Download as PowerPoint slideHighlights► Tombusviruses recruit the yeast Dbp2 DEAD-box helicase. ► Dbp2 is a homolog of human p68 DEAD-box helicase. ► Dbp2 binds to the 3′-end of the viral minus-stranded RNA. ► Dbp2 enhances plus-strand synthesis by the viral replicase. ► Dbp2 complements Ded1 DEAD-box helicase, another host factor involved in TBSV replication.